| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Martorello, Gianna M (10)
|
MRWEST |
2:31.93 |
2:30.68 |
-1.25 |
| 2 |
Gonzalez, Emilianna (10)
|
MRAPEX |
|
2:44.46 |
- |
| 3 |
Garretson, Phoebe N (10)
|
MRPAC |
3:14.77 |
2:59.31 |
-15.46 |
| 4 |
DeCasseres, Brianna O (7)
|
MRWEST |
3:15.59 |
3:09.04 |
-6.55 |
| 5 |
Klein, Mikayla (9)
|
MRWEST |
|
3:17.21 |
- |
| 6 |
Cioffi, Fiamma (9)
|
MREAST |
3:30.00 |
3:18.48 |
-11.52 |
| 7 |
Cabello, Mackenzie E (9)
|
MREAST |
3:35.00 |
3:21.43 |
-13.57 |
| 8 |
Johnsen, Sasha T (9)
|
MRSMC |
3:20.99 |
3:22.32 |
1.33 |
| 9 |
Iacobazzi, Alba (9)
|
MREAST |
3:30.00 |
3:23.94 |
-6.06 |
| 10 |
Li, Summer A (9)
|
MRAPEX |
|
3:24.47 |
- |
| 11 |
Otis, Luella K (10)
|
MREAST |
3:33.00 |
3:27.02 |
-5.98 |
| 12 |
Reis, Sophie M (8)
|
MREAST |
3:45.00 |
3:27.88 |
-17.12 |
| 13 |
Weinstein, Mary J (8)
|
MRWEST |
3:35.50 |
3:35.78 |
0.28 |
| 14 |
Donahue, Angelina H (9)
|
MREAST |
3:58.22 |
3:46.55 |
-11.67 |
|
Diaz Onderwyzer, Juliet R (9)
|
MREAST |
3:35.00 |
DQ |
- |